major outer membrane protein meaning in Chinese
主要外膜蛋白
Examples
- The major outer membrane proteins ( momps ) of an aeromonas hydrophila named ahl316 which isolated from diseased eel and the referential strain ahtps - 30 were purified and used for preparing immunostimulating complexs ( momp - iscoms ) . the immunotrial was carried out by injecting european eel peritoneally with 20ug of momp - iscoms per eel
本研究选取鳗源嗜水气单胞菌l316和参考菌株嗜水气单胞菌tps - 30 ，分别制备了这两株菌主要外膜蛋白免疫刺激复合物( momp - iscoms ) 。 - Make all these together , it proved that the cloned gene represented the major outer membrane protein gene of ahl316 , and the expressed gene products shared identical antigenicity with the natural main outer membrane protein . the studies on preparation and application of momp - iscoms of ah l316 provided a new approach to fish vaccinology . the successfully cloning and expressing the major outer membrane protein gene of ah l316 made it possible to describe this gene ' s function under a single factor level , and also provided technical support for developing an advanced gene engineering vaccine and subunit vaccine against aeromonas hydrophila
鳗源嗜水气单胞菌l316主要外膜蛋白免疫刺激复合物的制备与应用研究，对研制鱼类疫苗学问题进行了新的初步探索；成功地克隆和表达嗜水气单胞菌l316主要外膜蛋白基因为在单因子水平上研究嗜水气单胞菌外膜蛋白的作用和免疫功能以及制备嗜水气单胞菌基因工程疫苗和亚单位疫苗奠定技术基础。 - The homologious comparison proved the cloned gene had 96 % homology to the sequence of the omp gene , and the alignment of the amino acid sequence was 98 % . the recombinant plasmid was constructed with the target gene and the expressing vector pgex - 4t - l and then was transformed into e . coli bl21 ( de3 ) the fusion protein was expressed under the iptg inducing condition , and exhibited about 62kda in size , very close to the predicted molecular weight of gst - momp . furthermore , the fusion protein was specifically recognized by anti - serum which raised against the major outer membrane protein of ahl316
Sds - page电泳分析显示诱导表达的基因产物分子量约为62kda ，与预测的gst -外膜蛋白重组融合蛋白的分子量极为相似， western - blot进一步证实，表达产物能被嗜水气单胞菌l316主要外膜蛋白特异性抗血清所识别，产生明显的染色条带，说明所表达的基因产物与天然的外膜蛋白抗原性一致。